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DNA sequencing simulation

The diagram below shows a gel electrophoresis plate which has been used to separate DNA fragments produced as a result of the process described in Sequencing the DNA. All of the DNA fragments started at the bottom of the plate. The current during electrophoresis flowed in such a way that they were carried UP the plate, in the direction of the dotted red arrow.

Use your knowledge of DNA sequencing together with the information from the electrophoresis to work out the sequence of the original strand of DNA, starting at the 5' end. Enter your answers using the radio buttons under the diagram. Click on the button at the bottom of the page to see if you have worked out the sequence correctly.

gel electrophoresis

  5' original DNA strand
 
                                                         
3'
A    
C    
G    
T    

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