The evolution of strains of bacteria that are not affected by a particular antibiotic as a result of natural selection.
Viral enzyme in retroviruses that transcribes viral RNA into a DNA strand that can be used to direct the production of new virus particles.
Enzymes produced by certain bacteria which cut DNA at specific sites. They are widely used in genetic engineering.
The membrane which forms the boundary between the cytoplasm of a cell and the medium surrounding it and controls the movement of substances into and out of the cell.
An enzyme which is involved in DNA replication by catalysing the formation of phosphodiester bonds.
This is a gene added in the process of genetic engineering along with the gene for a desired characteristic. The marker gene usually codes for a feature such as fluorescence or the ability to synthesise a specific nutrient, which enables scientists to identify successfully engineered organisms when it is expressed.
Medicine that acts against bacterial infections. Penicillin is an example of an antibiotic.
Cells that make up animals, plants, fungi and protista. They are three-dimensional, membrane-bound sacs containing cytoplasm, a nucleus and a range of membrane-bound organelles.
A micro-organism that causes disease.
A small circle of extra DNA found in bacteria
Reusable protein molecules which act as biological catalysts, changing the rate of chemical reactions in the body without being affected themselves
An agent that carries a pathogen from one organism to another. In genetics, a vector is a virus used to transfer genetic material into a cell
The person who donates an organ for a transplant operation
The smallest of living organisms. Viruses are made up of a ball of protein that contains a small amount of the virus DNA. They can only reproduce after they have infected a host cell
An organism that is genetically identical to its parent.
The basic unit from which all living organisms are built up, consisting of a cell membrane surrounding cytoplasm and a nucleus.
The molecule which transcribes the DNA code and carries it out of the nucleus through the pores in the nuclear membrane to the ribosomes in the cytoplasm which synthesise the required proteins
In the early 1970s, the first genetically engineered organism was a bacterium - E. coli. The techniques used for genetic engineering are changing all the time. The basic principles of classic genetic engineering are shown here. The recently developed CRISPR-Cas9 gene editing process is covered later.
Stage 1: The desired gene can be removed from the DNA of the donor organism using enzymes called restriction endonucleases. These are enzymes which chop up DNA strands by cutting them at specific sites, so they can be used to remove very specific genes. Certain types of restriction endonucleases are particularly useful because they leave small regions of DNA sticking out at each end of the required gene. These are known as sticky ends, and make it much easier to attach the gene into another piece of DNA.
Sometimes the required gene is synthesised artificially. Using another specialised enzyme known as reverse transcriptase, the DNA sequence of the gene can be built up from isolated pieces of mRNA which have already been transcribed from the required gene.
Stage 2: The second step is to prepare a vector molecule to carry the DNA into the host cell – often a bacterium. A bacterial plasmid (a small circular strand of DNA often found in bacteria in addition to their main DNA) is often used as a vector. These replicate quickly and independently of the main bacterial genome, and can therefore amplify the number of copies of the gene.
Plasmids usually carry a marker gene which is used to demonstrate the cells which have been successfully engineered. In the early days, these marker genes often coded for characteristics such as resistance to a particular antibiotic. However, there were many concerns about the use of antibiotic resistance as marker genes, including the risk of them crossing into pathogens. In response, scientists modified their techniques and now other characteristics, such as fluorescence or the ability to synthesise a specific nutrient are used to identify the genetically modified organisms. So, for example, bacteria can be grown in a medium with a particular nutrient missing to show which cells have been successfully engineered: only the genetically-engineered organisms will be able to synthesise the missing nutrient and so only they will grow.
The bacterial plasmid is opened up using restriction enzymes which leave sticky ends that correspond to those of the new gene.
Stage 3: The third step is to join the new gene into the bacterial plasmid. The sticky ends are lined up and the gene is attached or annealed – using enzymes called DNA ligases which join the pieces of DNA together.
Stage 4: The final step is to incorporate the engineered DNA into the bacterium or other cell where it is required. This is known as transformation and is usually achieved by suddenly heating up the bacteria which makes their cell membrane more permeable so plasmid can move into the cells. Once the plasmid is inside the host bacterium it will be expressed and a new protein made.
Microorganisms are the most commonly used organisms in genetic engineering because they are relatively easy, quick and cheap to culture and there are few ethical issues about their usage. This entire process can either be used to generate a genetically engineered product, or as just a step of the genetic engineering process to clone the DNA (make many copies of it) before it is studied in more detail and introduced into a more complex organism.
To change the DNA of eukaryotic organisms such as people, a different vector must be used (usually a virus as human cells don’t contain plasmids), the correct cells must be targeted, the gene must be activated once it is inside the cell, the gene must be integrated correctly, and all these things must occur without harmful side effects. Engineering eukaryotic cells is often more complex than modifying bacteria, but as techniques develop it is becoming increasingly common.