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Topic last updated: 30 Sep 2021
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Diabetes 16+

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Discovery of insulin

In 1921 two researchers, Fred Banting and Charles Best, were the first to discover insulin and use it to treat diabetes.

Animal testing

At the time that Banting and Best were looking for a cure for diabetes, there were no alternatives but to perform their experiments using animals.

To test their theory Banting and Best used 10 dogs. They made the dogs diabetic and then investigated treatments for the diabetes. The result of their research has saved the lives of millions of people.

Injecting insulin allows
individuals with diabetes to
control their blood sugar levels.

Banting and Best's discovery of insulin

 

Some people have very strong opinions about testing medicines on animals. Today the use of animals is very tightly regulated and only allowed in circumstances where there are no other viable alternatives. Decide on a set of rules that should govern whether testing can take place or not.

Click here to see what the current UK law says about animal research.

Using animals for biomedical research is highly regulated in the UK. The law contains these principles:

  • The required information cannot be obtained using non-animal methods.
  • The likely benefits of doing the tests must outweigh any possible distress to the animals.
  • The test should use the minimum number of animals, appropriate species and cause the minimum distress.
  • Proper veterinary care should be given at all times.
  • Non-animal alternatives should be used wherever possible.
  • The person doing the test needs to be fully trained. Permission from the Home Office must be given before the study can begin.
  • The location for the tests must have all the necessary staff and facilities to ensure that animals are properly looked after.
  • Inspectors regularly monitor the locations where tests are being performed to check that all requirements are being met.

 

Insulin

Banting and Best did not know the chemical structure of insulin. In 1955, Fred Sanger determined its amino acid structure and in 1969, Dorothy Hodgkin used X-ray crystallography to find its three-dimensional structure.

The insulin molecule acts by attaching to cell-surface receptors on its target cells. Its three-dimensional structure enables it to attach to these receptors. The shape of the insulin molecule is determined by the way the protein chains fold around each other due to hydrogen bonds and disulfide bridges.

Insulin is a protein made up of two amino acid chains.

Molecular model of insulin molecule (ribbon)

This model shows the three-dimensional structure of insulin. It is a complex protein hormone.

Image courtesy of: T. Blundell & N. Campillo / Wellcome Images

 

Protein structure

The three dimensional structure of protein is described using four categories:

  • Primary
    Amino acid sequence of the polypeptide chain. e.g. amino acids in insulin's A and B chains.
  • Secondary
    How the amino acid chain folds back onto itself. Typically beta sheets or alpha helices.
  • Tertiary
    Folds in the beta sheets or helixes to give overall structure of the polypeptide chain.
  • Quaternary
    Some proteins are made of more than one polypeptide chain. These are joined to make the functioning protein. e.g. insulin's A and B chains are joined by disulfide bridges.